ABSTRACT
ABSTRACT: Gallus gallus domesticus' immune system is a promising tool for generation of antibody-based immunobiologics. Immunoglobulin y (IgY) is extracted from egg yolk and has equivalent functions to mammal's igg antibody. Avian immune system can be stimulated to produce a high-quality antibody repertoire. In this review, we present an overview of avian immune system emphasizing igy and its applications as an immunobiologic.
RESUMO: O sistema imunológico deGallus gallus domesticus é uma ferramenta promissora para a geração de imunobiológico a partir de anticorpos. A imunoglobulina Y (IgY) é extraída da gema do ovo e apresenta funções equivalentes ao anticorpo IgG dos mamíferos. O sistema imune aviário pode ser estimulado para produzir um repertório de anticorpos de alta qualidade. Nesta revisão apresentamos aspectos gerais do sistema imune aviário enfatizando o IgY e suas aplicações como um imunobiológico.
ABSTRACT
A viabilidade e maturidade fetais podem ser estimadas através da avaliação dos fluídos fetais em muitas espécies, no entanto a composição bioquímica do líquido amniótico durante a gestação ainda não está bem definida para a espécie equina. O objetivo deste estudo foi estabelecer e comparar o perfil bioquímico do liquido amniótico em diferentes momentos da gestação e no momento do parto para um melhor entendimento da fisiologia da gestação de equinos. Foram avaliados valores encontrados para pH, osmolaridade, glicose, uréia, creatinina, gamma-GT, sódio, potássio, cloretos e proteína total no liquido amniótico colhido de 122 éguas comparando-se os resultados encontrados entre as colheitas em terço inicial (TI), médio (TM) e final (TF) da gestação e no momento do parto (MP). [...] Valores para gamma GT diferiram apenas entre os grupos TF e MP e mais estudos devem ser conduzidos sobre sua função no liquido amniótico das espécies domésticas. Os valores de sódio e cloretos não diferiram significativamente entre os grupos estudados e as concentrações de potássio diferiram apenas entre os grupos TF e MP refletindo a manutenção do equilíbrio eletrolítico do líquido amniótico equino durante a gestação. As concentrações de proteínas totais variaram de maneira heterogênea entre os grupos, porém todos estes demonstraram baixas concentrações. Concluímos que a composição bioquímica do liquido amniótico equino varia de acordo com o desenvolvimento fetal e pode ser utilizada como mensuração da viabilidade e maturidade do feto no futuro, no entanto devido ao pequeno número de estudos conduzidos e diferenças encontradas em seus resultados, outros estudos devem ser realizados para a melhor definição do perfil bioquímico do líquido amniótico equino durante as fases gestacionais e no momento do parto.
The viability and fetal maturity can be estimated by biochemical evaluation of the fetal fluids of several species; however the biochemical composition of amniotic fluid during pregnancy is not fully defined for equine. The aim of this study was to establish and compare the biochemical profile of amniotic fluid in different moments of pregnancy and at delivery, in order to better explain the peculiarities of the physiology of pregnancy in mares. The values founded for pH, osmolarity, glucose, urea, creatinine, gamma-GT, Sodium, potassium, chloride and total protein were evaluated in amniotic fluid collected from 122 mares comparing the results between the initial-third (IT), mid-third (MT) and latter-third (FT) of gestation and at delivery (D). [...] Concentrations of urea tended to be statistically different in at least one of the groups. A significant increase in creatinine concentrations was observed during the initial-third, medium-third and the final-third of pregnancy and the value found at delivery remained equal to final-third. Values for Gamma GT differed only between FT and D groups and more studies should be conducted about its role in the amniotic fluid of domestic species. For the sodium and chloride ions, they were not significantly different between the studied stages, and the potassium ion was significantly different only between FT and D reflecting the maintenance of electrolyte balance of the amniotic fluid during equine pregnancy. Total protein concentrations were different between groups, but all the groups showed low concentrations. We conclude that the values of the parameters studied varied according with the fetal development and can be used for the evaluation of fetal viability and maturity in the future, but the scarce number of studies in this area and the difference between the results found in literature demonstrate the need of more works to establish the biochemical profile of equine amniotic fluid during pregnancy and at delivery.
Subject(s)
Animals , Female , Horses/physiology , Gestational Age , Pregnancy/physiology , Amniotic Fluid/chemistry , Parturition/metabolism , Fetal Organ Maturity , Fetal ViabilityABSTRACT
Introduction :Without uniform recognition of Emergency Medicine as a specialty in developing sub-Saharan African countries; data are limited on the epidemiology of emergency care needs. The purposes of this study were to quantify the burden of disease presenting as medical or surgical emergencies and describe the patient population at a small community medical centre in the Republic of Tanzania. Methods : An observational study was conducted from March to June 2011 at the University of Arusha (UOA) Medical Centre in Arusha; Tanzania. All consenting patients presenting with acute illness or injury were eligible for inclusion in the study. A standardized form was used to record demographic information; chief complaint; diagnosis/diagnoses; procedure(s); treatment(s); and disposition. Results : Data were collected on 719 patients; with a mean age of 21.8 years (range neonate to 83 years). Fever (19.5); respiratory (17.5); and gastrointestinal complaints (15.0) were the top three chief complaints; 94.9 of chief complaints were atraumatic. The top three diagnoses included respiratory infection (22.1); malaria (21.4); and skin or soft tissue infection (7.9). Forty-three percent of patients required no procedures or tests; and 42 required only one procedure or test. Of treatments administered; 67.6 were analgesics; 51.3 were cough medications; and 47.6 were antipyretics. Ninety-seven percent of patients were discharged home after their visits. Discussion: Respiratory infections; malaria; and skin or soft tissue infections are leading reasons for seeking medical care at a small community medical centre in Arusha; Tanzania; highlighting the burden of infectious diseases in this type of facility. Males may be more likely to present with trauma; burns; and laceration injuries than females. Many patients required one or no procedures to determine their diagnosis; most treatments administered were inexpensive; and most patients were discharged home; suggesting that providing acute care in this setting could be accomplished with limited resources